蛋白酶K(冻干粉)
- 别名:Proteinase K;Endopeptidase K; Prok; Protease K;39450-01-6
- 编码:EBY0027I
- 级别:≥30 Units/mg protein
- 简介:蛋白酶K是一种枯草蛋白酶类的高活性蛋白酶,用于生物样品中蛋白质的一般降解。蛋白酶K是一种切割活性较广的丝氨酸蛋白酶。它切割脂族氨基酸和芳香族氨基酸的羧基端肽键。此酶经纯化去除了RNA酶和DNA酶活性。由于蛋白酶K在尿素和SDS中稳定,还具有降解天然蛋白质的能力。
基础信息
中文名 | 蛋白酶K(冻干粉) |
英文名 | Proteinase K, lyophilized powder |
CAS | 39450-01-6 |
E.C | 254-457-8 |
Mol. Weight | 29KDa |
Source | Tritirachium album limber(林伯氏白色念球菌) |
产品介绍
蛋白酶K是一种枯草蛋白酶类的高活性蛋白酶,用于生物样品中蛋白质的一般降解。蛋白酶K是一种切割活性较广的丝氨酸蛋白酶。它切割脂族氨基酸和芳香族氨基酸的羧基端肽键。此酶经纯化去除了RNA酶和DNA酶活性。由于蛋白酶K在尿素和SDS中稳定,还具有降解天然蛋白质的能力。
蛋白酶K用途
- 基因诊断试剂盒
- 基因组DNA提取试剂盒
- RNA提取试剂盒中去除DNA和RNA制备中的核酸酶
- 提取组织中非蛋白成份降解含有蛋白质的杂质-譬如DNA疫苗和肝素的制备
- 制备脉冲电泳的染色体DNA
- 蛋白质印迹
- 蛋白酶K的一般工作浓度50-100ug/ml
蛋白酶K参考文献资料
1. 【产品推荐】蛋白酶K (源自林伯氏白色念球菌) Proteinase K,from Tritirachium album limber
2. Kraus, E; et.al. Proteinase K from the Mold Tritirachium album limber, Specificity and Mode of Action. Z. Physiol. Chem., 357:939; 1976.
3. Jany,KD, et al. Amino Acid Sequence of Proteinase K from the Mold, Tritirachium album limber. Proteinase K; a Subtilisin-related Enzyme with Disulfide Bonds. FEBS Letter, 199,139.1986.
4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Volume 3, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, B.16.
5. Sweeney, P.J. and Walker, J.M. (1993) Enzymes of molecular biology. In: Methods in Molecular Biology, Vol. 16, M.M. Burrell, ed., Humana Press, Inc., Totowa, NJ, 305.
2. Kraus, E; et.al. Proteinase K from the Mold Tritirachium album limber, Specificity and Mode of Action. Z. Physiol. Chem., 357:939; 1976.
3. Jany,KD, et al. Amino Acid Sequence of Proteinase K from the Mold, Tritirachium album limber. Proteinase K; a Subtilisin-related Enzyme with Disulfide Bonds. FEBS Letter, 199,139.1986.
4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Volume 3, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, B.16.
5. Sweeney, P.J. and Walker, J.M. (1993) Enzymes of molecular biology. In: Methods in Molecular Biology, Vol. 16, M.M. Burrell, ed., Humana Press, Inc., Totowa, NJ, 305.
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质量指标
Activity(37℃) | ≥30.0U/mg |
Solubility | Clear |
DNase | Not detected |
RNase | Not detected |
Appearance | White lyophilized powder |
MSDS
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